Mouse anti-Human IgE Antibody (Biotin)

Catalog No. ABIN457503

Quick Overview for Mouse anti-Human IgE Antibody (Biotin) (ABIN457503)

Target: IgE

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Clone: BSW17-NBS01

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes

Sensitivity: Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)

Characteristics: IGHE, chromosome 14q32.33

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck

Purity: > 85 %, tested by analytical Gel Permeation Chromatography

Sterility: Sterile filtered

Immunogen: purified human myeloma IgE

Application Details

Application Notes: Sterile filtered solution, does not contain Na-azide. Recommended for functional cellular assays (mast cell / basophil activation).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Preservative: Azide free

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C

Target Details

Target: IgE

Background: The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.